The list was voted for by over 2,000 gamers through onepoll.com.
‘Old skool’ titles such as Pac Man, Donkey Kong and Frogger did particularly well in the list.
John Sewell, spokesman for www.onepoll.com, said: "Games which were invented years ago, like Space Invaders, Donkey Kong, Super Mario and Pac Man all appear in the top ten.
"This shows that classic games will live on forever in the hearts of computer game fans.
"The gaming industry is getting more and more competitive, releasing more and more innovative games by the minute - but this survey proves that sometimes the most simple formats work well to keep game players hooked."
The top five was made up of Super Mario Bros, Pac-Man, Tetris, The Sims and Sonic The Hedgehog.
According to The Telegraph, the full top 20 reads:
1. Super Mario Bros.
4. The Sims
5. Sonic the Hedgehog
6. Space Invaders
7. Grand Theft Auto - San Andreas
8. Donkey Kong
9. Mario Kart 64
10. Grand Theft Auto - Vice City
11. Lara Croft: Tomb Raider
12. Wii Sports
13. Final Fantasy
14. Gran Turismo
17. Pokemon, Red, Blue and Green
18. Street Fighter II
19. Wii Play
20. Guitar Hero